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KMID : 1145120170150020146
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Asian Journal of Beauty and Cosmetology
2017 Volume.15 No. 2 p.146 ~ p.158
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Anti-oxidant Efficacy and Effects on Expression of Growth Factors in Human Hair Follicle Dermal Papilla Cells of Rosa multiflora Root Extracts
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Hwang Hyeon-Sook
Hwang Tae-Hyun
Pyo Ae-Ja
Ju Eun-Hwa
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Abstract
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Purpose: The anti-oxidant activity of active ingredients extracted from Rosa multiflora root was evaluated and effects of cell proliferation and growth factor expression were confirmed.
Methods: Total polyphenol and flavonoid content of the active ingredients extracted from Rosa multiflora root was measured in various ways, as were 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and nitrite scavenging activity. Further, the cell proliferation effects for human hair follicle dermal papilla cell (HFDPC) were measured using thiazolyl blue tetrazolium bromide (MTT) assay, as were the effects of promoting the expression of growth factors such as platelet derived growth factor subunit B (PDGFB), keratinocyte growth factor (KGF), insulin like growth factor 1 (IGF1), vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), and epidermal growth factor (EGF) by Western blot.
Results: The total polyphenol content of six kinds of Rosa multiflora root extracts was the highest in supercritical extracts (101.925 mg/g) and the total flavonoid content was the highest in supercritical extracts (14.365 mg/g). DPPH scavenging activity was the highest in methanol-sonication extracts (98.24%) and nitrite scavenging activity was the highest in supercritical extracts (55.86%). HFDPC was treated with 10, 100, and 500 μg/mL of Rosa multiflora root methanol-sonication extracts to determine cytotoxicity. The cell growth rate increased with increasing concentrations. The effects of the expression of growth factor protein were 135% greater in IGF1 and 126% greater in PDGFB.
Conclusion: Rosa multiflora root extracts had no cytotoxicity but had excellent anti-oxidant ability. In addition, because the effects of promoting the expression of IGF1 and PDGFB were significant, it was confirmed that Rosa multiflora root extracts could be used as an antioxidant, functional cosmetic material, and as a natural material for hair restorer and a hair-growth promotion agent.
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KEYWORD
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Rosa multiflora, Human hair follicle dermal papilla cells, Western blot, Growth factor, MTT assay
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